The blood vasculature in the brain is a highly ramified, complex but well-organized vessel network. During development, the pathfinding of growing vessels is critical for the patterning of the brain vasculature. However, its underlying mechanism still remains elusive. 

A research team led by Dr. DU Jiulin of the Institute of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology of Chinese Academy of Sciences, has revealed that Ca²⁺ activities mediated by mechanosensitive Piezo1 channels regulate the pathfinding of growing brain vessels in larval zebrafish. 

The formation of the brain vasculature is initiated by vessel invasion from the surrounding perineural vascular plexus around the ventral neural tube during early development, and then the brain vasculature is expanded by the continuous emergence and growth of newborn vessels from preexisting ones, a process known as angiogenesis. 

Endothelial tip cells (ETCs) located at the forefront of growing vessels usually extend a few motile primary branches and many dynamical fine filopodia, navigate through tissue microenvironments, and steer angiogenic sprouts to their appropriate targets. This process of ETC pathfinding is critical for the proper patterning of the vasculature in the brain as well as in all other organs, but its underlying mechanism still remains largely unknown. 

To investigate the cellular and molecular mechanism underlying ETC pathfinding, Dr. DU's group first monitored the entire process of ETC pathfinding during brain vascular development through in vivo long-term time-lapse simultaneous imaging of both the morphological dynamics and Ca²⁺ activity of ETCs in larval zebrafish. 

They found that before reaching target vessels, ETCs frequently extended and retracted subcellular primary branches, leading to continuous changes in the direction of ETC migration and vessel growth. 

Interestingly, branches of ETCs exhibited spontaneous local Ca²⁺ transients at different frequencies during pathfinding, with high- and low-frequency local Ca²⁺ transients associated with branch retraction and extension, respectively. 

Then the researchers examined the causal relationship between local Ca²⁺ transients and fate determination of ETC branches via local manipulation of Ca²⁺ concentration at ETC branches, and found that high- and low-frequency Ca²⁺ transients were necessary and sufficient for the retraction and extension of ETC branches, respectively. 

Furthermore, they investigated the origin of local Ca²⁺ activities of ETCs, and discovered that mechanosensitive Piezo1 cationic channels were preferentially expressed on ETC branches and activated by tissue stiffness-associated mechanical force.  They mediated local Ca²⁺ activities of ETC branches, thus regulating the retraction and extension of ETC branches.   

Mutating piezo1 largely diminished local Ca²⁺ transients of ETC branches, impaired the pathfinding of ETCs, and therefore disrupted the patterning of the brain vasculature. 

Finally, the researchers showed that the protease calpain and nitric oxide synthase mediated the effects of Piezo1-mediated Ca²⁺ activities on ETC branch retraction or extension, respectively. 

This study reveals that Piezo1 and downstream Ca²⁺ signaling act as molecular bases for ETC pathfinding and highlights a novel function of Piezo1 and Ca²⁺ in vascular development.