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shared biomaterials

Time：2020-01-21

Plasmids from Article Homology-mediated end joining-based targeted integration using CRISPR/Cas9 DOI:10.1038/cr.2017.76

Plasmids in Addgene

Addgene ID	Name of Plasmid
97307	AAV_Efs_hSpCas9_NLS_FLAG-SV40
97308	AAV_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA
97309	AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA
97310	AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA
97311	AAV_Actb MMEJ donor_U6_sgRNA_EF1a_GFP_polyA
97312	Lenti_Actb HMEJ donor_U6_sgRNA_EF1a_GFP_polyA
97313	Lenti_Efs_hSpCas9_NLS_FLAG-WPRE
97315	AAV_Actb MMEJ donor
97316	AAV_Actb NHEJ donor
97317	AAV_Actb HR donor
97318	AAV_Actb HMEJ donor
97319	AAV_Cdx2 HMEJ donor
97320	AAV_Dbh HMEJ donor
97321	AAV_Nanog HMEJ donor
97322	AAV_Sox2 HMEJ donor

Plasmids from Article In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice DOI:10.1038/s41593-017-0060-6

Plasmids in Addgene

Addgene ID	Name of Plasmid
107307	CAG-LoxpStopLoxp-NLS-dCas9-HA-NLSNLS-10xGCN4-NLS-P2A-scFv-NLS-P65-HSF1-Flag-T2A-EGFP-WPRE-PolyA
107310	dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4
107311	EF1α-scFv-p65-HSF1-T2A-EGFP-WPREPolyA
107312	AAV-hSyn-mCherry-P2A-Cre-WPRE
107313	AAV-aCamkII-mCherry-P2A-Cre-WPREBGH-polyA
107314	dSV40-NLS-dCas9-HA-NLS-NLS-10xGCN4-P2A-mCherry

Mice from Article In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice DOI:10.1038/s41593-017-0060-6

	name	other called	URL
Stock No. at Jackson Lab 031645	B6;D2-Gm33925Tn(pb-CAG-cas9*,-EGFP)1Yangh/J	Cre-dependent SPH (SunTag-p65-HSF1)	https://www.jax.org/strain/031645
Stock No. at Shanghai Model Organisms NM-TG-00025	C57BL/6-Tg(CAG-LSL-dCas9-SPH)Smoc	CAG-LSL-dCas9-SPH	https://www.modelorg.com/portal/article/index/id/2261/post_type/3.html

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