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Behavioral setup

Linear track setup

VR setup

Fear conditioning setup

Neural recording and manipulation

Multi-channel single-unit recording

UCLA miniscope

Photometric recording

Patch-clamp recording

Juxtacellular recording

Optogenetics

Common setups

Fluorescence microscope

Stereotaxic injection

Molecular Cloning

Neural circuit tracing

Rabies based trans-synaptic tracing

Intersectional control

Behavioral setup

Linear track setup

·        Fully automatic control of behavioral training

·        Interleaved trials for context switches

·        Context-dependent tasks in behaving animals

VR setup

·        Rodent running on a spherical treadmill

·        270° TFT monitor (or 150° curved back-projecting screen) for presenting virtual reality

·        Custom behavioral training with licking reward and air-puff punishment

·        Compatible with 2-photo microscopy imaging

·        Compatible with miniscope Ca²⁺ imaging

Fear conditioning setup

·        Custom software to acquire behavioral video and trigger optogenetic devices

·        Compatible with photometry and miniscope recording

Neural recording and manipulation

Multi-channel single-unit recording

·        64 channel acquisition systems from BlackRock and Open-Ephy

·        Tetrode recording in free-moving animals

·        Spike sorting using OFSS

UCLA miniscope

·        > 1mm diameter field of view and ~ 1mm working distance

·        +/- 200um electronic focal adjustment

·        2.6 grams and 22 mm tall

·        Absolute head orientation sensor

(Courtesy of Miniscope-v4 wiki. We are using v2 in the lab for now)

Photometric recording

·        Multi-Fiber Photometry System (4 channels)

·        Integrated with behavioral video acquisition and analysis

·        Synchronized with external equipment (such as optogenetics) via TTL

Patch-clamp recording

·        Axon 700A/700B, Digidata 1440, pClamp10.

·        Sutter Motorized Micromanipulator, Nikon microscope, Thorlab CCD.

Juxtacellular recording

·        npi amplifier ELC-03XS, CED Micro 1401

Optogenetics

·        462 nm diode blue laser, 589 nm DPSS yellow laser

·        Adjustable power, TTL trigger mode

Common setups

Fluorescence microscope

·        Blue, Green, Red and Far-red channels

·        Equipped with motorized platform and NeuroLucida 8

Stereotaxic injection

·        Digital display of stereotaxic coordinates

·        Precise control of isoflurane

·        Fast anesthesia and fast recovery

Molecular Cloning

·        Standard equipment for cloning

Neural circuit tracing

Rabies based trans-synaptic tracing

·        Cell-type specific retrograde trans-synaptic tracing

·        Example below showing projection-cell-specific tracing of vHC-CEA-PAG pathway

·        Quantitative analysis of circuit tracing in the whole-brain scale

Intersectional control

·        IBIST (intein based intersectional synthesis of transactivator)

·        Intersectional expression for ChR2 and GCamP6 in tTA-dependent manner