Gene-edited Disease Monkeys Cloned in China

Time:2019-08-06

Gene-edited Disease Monkeys Cloned in China

  The first cohort of five gene-edited monkey clones made from fibroblasts of a monkey with disease phenotypes were born recently at the Institute of Neuroscience (ION) of Chinese Academy of Sciences (CAS) in Shanghai. The expression of BMAL1, a core circadian regulatory transcription factor, was knockout in the donor monkey using CRISPR/Cas9-mediated gene editing at the embryo stage, and the fibroblasts of the donor monkey were used to clone five monkeys using the method of somatic cell nuclear transfer, the same method that generated Zhongzhong and Hua Hua, the first two cloned monkeys,last year. This major advance, reported on-line in two articles in the journal National Science Review on January 24, demonstrates that a population of customized gene-edited macaque monkeys with uniform genetic background will soon be available for biomedical research.

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